DNA Preparation & Mouse Genotyping
ABR, in conjunction with Garvan Molecular Genetics (GMG), offers a quick and convenient genotyping service for our clients. ABR does the tissue collection and DNA extraction and GMG undertakes the genotyping. With a typical turnaround time of 4-5 business days, this service is increasingly popular, currently handling around 4,000 samples each month.
GMG require all new projects to complete and submit a New Project Submission Form in order to set up the genotyping process. Where the line is new to ABR through an import process or a new cross of lines a Line Information Form is required.
For mice at ABR: Tissue collected by ABR technicians is placed in a barcoded tube and sent to the ABR laboratory, where it is processed using a semi-automated, high throughput method. The extracted, purified DNA is then sent to Garvan Molecular Genetics for High Resolution Meltcurve (HRM) analysis.
Samples can be tracked as they progress through each stage, as results are automatically uploaded into the Stuart animal management software.
For mice external to ABR: Samples from animals not housed at ABR can also be handled through this service. Results will be emailed directly to the client. Please refer to the Sample Submission Guidelines on the GMG webpage for future information about preparing samples and use the Sample Submission Form when sending samples.