Genome Editing - The MEGA Service
Mouse Engineering at Garvan/ABR (MEGA) offers an integrated service to generate novel GM mouse lines quickly and cost-effectively using CRISPR/Cas9 technology.
CRISPR/Cas9 technology produces rapid, targeted genetic modifications including single nucleotide mutations, constitutive knockouts or insertions.
Advantages of CRISPR/Cas9 Technology
- Rapid and low cost (5 to 10-fold faster and cheaper than traditional ES cell approach)
- Simplier and more efficient than TALENs and Zinc Finger Nuclease
- Can target multiple genes simultaneously to reduce breeding and housing costs
The comprehensive service includes project consultation, strategy design, sgRNA and Cas9 mRNA preparation, microinjection, embryo transfer and production of founder mice. Pups are screened at 2-3 weeks of age for the presence of the genetic modification.
In addition, ABR can receive DNA constructs designed and prepared by your research group for standard pronuclear injection.
Project Level and Pricing
Level 1 Project ($9,302.70): targeting one or two sgRNAs to a specific locus to introduce small (1-20bp) insertions/deletions or larger deletions (0.1-5.0kb). In the absence of adverse phenotypes, production and identification of founder mice from Level 1 projects (gene KO or large deletions) can take as little as 8-12 weeks from placement of the order. Up to 4 genes can be simultaneously targeted.
Level 2 Project ($12,376.10): incorporating small changes (e.g. point mutations, small specific insertions/deletions up to 60bp) via sgRNA-directed gene targeting and homologous recombination with a single-stranded DNA oligonucleotide substrate (~150 bases). The minimum time for Level 2 Projects is 10-14 weeks due to the synthesis of targeting oligonucleotide.
Level 3 Project ($18,607.60): coupling of the targeting of one or two sgRNAs to a specific locus with subsequent homologous recombination with a plasmid DNA substrate (5-10kb) to either “flox” an exon or “knock-in” a reporter or other large piece of sequence.