The MEGA service can generate new mouse lines quickly and cheaply using CRISPR/Cas9 technology
Mouse Engineering at Garvan/ABR (MEGA) offers an integrated service to generate novel GM mouse lines quickly and cost-effectively using CRISPR/Cas9 technology.
CRISPR/Cas9 technology produces rapid, targeted genetic modifications including single nucleotide mutations, constitutive knockouts or insertions.
Advantages of CRISPR/Cas9 Technology
In addition, ABR can receive DNA constructs designed and prepared by your research group for standard pronuclear injection.
Project Level and Pricing
Level 1 Project ($9,302.70): targeting one or two sgRNAs to a specific locus to introduce small (1-20bp) insertions/deletions or larger deletions (0.1-5.0kb). In the absence of adverse phenotypes, production and identification of founder mice from Level 1 projects (gene KO or large deletions) can take as little as 8-12 weeks from placement of the order. Up to 4 genes can be simultaneously targeted.
Level 2 Project ($12,376.10): incorporating small changes (e.g. point mutations, small specific insertions/deletions up to 60bp) via sgRNA-directed gene targeting and homologous recombination with a single-stranded DNA oligonucleotide substrate (~150 bases). The minimum time for Level 2 Projects is 10-14 weeks due to the synthesis of targeting oligonucleotide.
Level 3 Project ($18,607.60): coupling of the targeting of one or two sgRNAs to a specific locus with subsequent homologous recombination with a plasmid DNA substrate (5-10kb) to either “flox” an exon or “knock-in” a reporter or other large piece of sequence.