Level 1: Gene KOs via small frameshift deletions or larger deletions up to 5kb.
Level 2: Small, specific edits using homologous recombination with ssDNA oligonucleotides (e.g. point mutations, peptide tags).
Level 3: Larger alterations using homologous recombination with plasmid constructs (e.g. reporters, fluorescent protein tags, floxing).
The prices for these services are $7,000, $10,000 and $15,000 respectively. The time-frame from order to identification of founder mice is now as low as 10 weeks for Level 1 and Level 2 projects.
Altogether, the CRISPR/Cas9 approaches offered by the MEGA (Mouse Engineering Garvan/ABR) service are 5-10 times cheaper and faster than conventional ES cell approaches, produce mice on a pure C57BL/6 (or FVB/N) background and are free of additional drug selection markers.
