Simultaneous Production of KO mice can now reduce costs to ~$3,000 per line

The efficiency of CRISPR/Cas9 gene targeting means that multiple genes can be simultaneously inactivated. The MEGA (Mouse Engineering Garvan/ABR) service has now undertaken projects in which founder groups carrying 2, 3 and 4 inactivated genes have been generated from a single microinjection session.

Because microinjection and animal husbandry are our major cost, MEGA is able to offer the following prices on Level 1 projects in which multiple genes will be simultaneously targeted for inactivation.

1 gene = $7,000
2 genes = $8,500 ($4,250 per KO)
3 genes = $10,000 ($3,333 per KO)
4 genes = $11,500 ($2,875 per KO)

As well as being the best way of producing novel KO lines, the economies from simultaneously producing multiple KO lines makes CRISPR/Cas9 a much cheaper and efficient option than importing KO lines into ABR, especially if rederivation is required.

If you would like to take advantage of the multiple targeting option, please get in touch with either Dr David Zahra at d.zahra@garvan.org.au or Professor Rob Brink at r.brink@garvan.org.au.